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Animal Behavior Research

Sleep deprivation system

来源:本站 作者:玉研仪器 Time: 2017-07-04 Source: Site Author: Jade research instrument

Detailed introduction

The sleep deprivation system for rats and mice is an instrument that does not require animals to be trained, and allows them to sleep in a gentle manner.   进行睡眠 剥夺的实验 After the parameters are set, experiments on sleep deprivation in rats or mice can be performed without human intervention .



Feedback 和StandAlone The system is available in two models: Feedback and StandAlone


Stand Alone type



Can be controlled by computer and controller touch screen;

The striking rod rotates in the squirrel cage at a speed of 5-15 RPM, and can be programmed to change direction to reduce the adaptation of the animal with sleep deprivation to the environment;


The system can provide food, water and sleeping places, animal cages are divided into rat cages and mouse cages;




Feedback type



EEG/EMG 脑电肌电系统实时监测的脑电肌电信号来进行睡眠剥夺,系统可以设置好动物的睡眠状态,当实时监测的脑电肌电信号与预设置的睡眠状态匹配或相似时,系统控制击打棒转动; Sleep deprivation can be performed through the EEG / EMG EMG signals monitored in real time by the EEG / EMG system. The system can set the sleep state of the animal. When the EEG signals monitored in real time match or are similar to the preset sleep state At the time, the system controls the rotation of the striking rod;

Controlled by computer and controller touch screen;

5-15 RPM 的速度转动,可以通过程序编制改变方向,以减少睡眠剥夺的动物对环境的适应; The system controls the striking stick according to the real-time monitoring of the EEG signal. The striking stick will rotate at a speed of 5-15 RPM. The direction can be changed by programming to reduce the adaptation of the animal with sleep deprivation;

The system provides food, water and sleeping places. Animal cages are divided into rat cages and mouse cages;


 





睡眠剥夺系统, STAND ALONE 睡眠剥夺系统 FEEDBACK sleep deprivation system, STAND ALONE sleep deprivation system

提供两种形式的自动睡眠剥夺系统: STAND ALONE 睡眠剥夺系统和 FEEDBACK 睡眠剥夺系统。 We offer two forms of automatic sleep deprivation system: STAND ALONE sleep deprivation system and FEEDBACK sleep deprivation system.

睡眠剥夺系统根据实时采集到动物的 EEG EMG 信号作为依据,反馈控制旋转杆的动作。 The FEEDBACK sleep deprivation system feedback-controls the movement of the rotating rod based on the animal's EEG and EMG signals collected in real time .

FEEDBACK SLEEP DEPRIVATION SYSTEM

FEEDBACK SYSTEM The FEEDBACK SYSTEM   provides calendar-scheduling functionality plus the capability of adding real-time EEG / EMG feedback to ensure the bar rotates only when the animal enters a sleep-like state. Bar rotation starts and stops automatically based on user established rule sets for the animal's sleep state , and users can easily incorporate delays, shifts in bar rotation, and time restrictions into the experimental setup.


The Feedback system can be controlled by a computer or by the programmable interface on the front panel of the device. Operating in feedback mode requires a computer connection.

  • The bar rotates at speeds between five and fifteen RPM and can be programmed to change directions to reduce acclimatization to the sleep deprivation environment. Bar rotation can be completely controlled by real-time EEG and EMG activity.
  • System accommodates water, food, and bedding. A variety of cage sizes are availble for mice and rats.
  • A Pinnacle EEG / EMG system is required to use EEG & EMG signals to regulate sleep deprivation.   See the "Rules / Scheduling" tab below for more information.
  • Optional video recording provides a platform for synchronizing EEG and EMG changes with observable behavioral states. Captured video is displayed on screen and is synchronized with other recorded data in playback mode.  

RULES-BASED PROGRAMMING

The true versatility of the feedback system is unlocked in Sirenia® Acquisition with the included license key. Our software uses EEG and EMG data to establish thresholds, or rule sets, for the animal's sleep patterns. Rule criteria can be easily adjusted by the researcher to target specific sleep behaviors and the system can be programmed to operate only during user-defined periods of time.

 

YOKED CONTROL FUNCTIONALITY

Add additional units to perform yoked control sleep studies. All systems can be programmed to operate simultaneously under one schedule or set of rules. A feedback system is required for yoked control.

EFFECTIVENESS OF YOKED CONTROL SLEEP DEPRIVATION

EEG and EMG activity were recorded for 24 hours (baseline) from two cohorts of five male C57BL / 6J mice. During the following 24 hours, the first cohort was actively sleep deprived (SD) for a 12-hour lights-on / 12- hour lights-off period. An identical cohort of five mice was subjected to yoked control (YC) sleep deprivation. The YC mice underwent the exact same sleep deprivation stimuli and duration as SD animals but were otherwise allowed to sleep ad lib. The chart below shows total cumulative sleep loss accrued during the 24-hour sleep deprivation period for both cohorts. Sleep loss was calculated by subtracting the total minutes of sleep accumulated during sleep deprivation from the total minutes of sleep accumulated during the same period during baseline. Purple bars represent the SD cohort, and gold bars represent the YC cohort. Vertical bars represent the standard error of the mean.

 

A Pinnacle   to operate the sleep deprivation unit in feedback mode. Pinnacle offers three- and four-channel systems for biopotential recording. Tethered solutions are available for both mice and rats. EEG / EMG system is required to operate the sleep deprivation unit in feedback mode. Pinnacle offers three- and four-channel systems for biopotential recording. Tethered solutions are available for both mice and rats.

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