⎝⎛七乐彩⎞⎠

Shanghai Yuyan Scientific Instrument Co., Ltd.

Phone: 021-35183767
021-54377179

FAX: 021-35183767-8008

QQ: 3007536621

Address: XingMei Rd 485, Shanghai, China, No.485 Xingmei Road, Minhang District, Shanghai

Current position: ⎝⎛七乐彩⎞⎠ > Physiological Signal Measurement> EEG Recorder
Physiological signal measurement

EEG recorder for rats and mice

来源:本站 作者:玉研仪器 Time: 2016-09-28 Source: Site Author: Jade research instrument

Detailed introduction

We can provide three-channel and four-channel systems for studying sleep, epilepsy, and behavioral patterns in mice and rats under free movement. The EEG / EMG system uses a unique head-mounted preamplifier to obtain a very clear waveform even when the animal is in motion. The four channels have the characteristics of all three channels and an additional channel is added. The ability to configure biosensors at the same time.


  • For real-time monitoring of animal brain electricity, myoelectricity, and chemical substance concentrations (glutamic acid, glucose, lactic acid, ethanol, choline, total biogenic amines)
  • Real-time monitoring of animals in free movement
  • Realize signal telemetry function, animals can be placed in the maze to monitor the concentration of chemicals in the brain in real time

The electroencephalograph of rats and mice can provide a set of professional bioelectric recording system for the study of sleep, epilepsy and general behavior paradigm of rats and mice under free movement. Using a headstage fixed to the animal's head for signal amplification, it can continuously record the ideal signal with extremely low noise even when the animal is moving.

We provide a pre-configured three-channel research system for sleep or epilepsy research. At the same time, a more flexible four-channel system can be provided, which integrates the biological probe for simultaneous recording of multiple signals. All systems support the optogenetics suite. Common applications: sleep research, epilepsy research, deep electrode recording, cortical recording, field potential, cognitive research

Some components:
  • Head holder


The prefabricated rat and mouse head holders fix the plugs connected to the EEG or EMG electrode wires to the micro board. The extra two needle electrodes are used in a 4-channel EEG configuration. Prefabricated fixture components reduce surgical time and allow multiple arrangements of electrodes, providing a plug-and-play wire connection.  

  • Preamplifier


The front put is secured to the animal's head with a screw.
High-pass filtering: 0.5Hz EEG, 10Hz EMG (sleep study); or 1.0Hz EEG, 10Hz EMG (epilepsy)
Gain: X100 (sleep), X10 (epilepsy)

  • Cable system


An 18-inch cable is connected to the preamp via a wheel. The outer layer of the wires in the cable is protected by a layer of metal spring wire.

  • Winding caster


An anti-winding wheel is fixed on the top of the animal cage. The two plugs of the runner are arranged to allow the movable lever to be balanced in different directions.

  • Data Acquisition and Analyzer

A data modulation and acquisition device (DCAS) provided by the system provides secondary amplification, filtering, and digitization functions. Finally, the data is transmitted to the computer running the acquisition analysis software via a USB cable.

Technical Parameters 3 channels 4 channels
Sampling rate 200Hz-2KHz 200Hz-20Hz
Low-pass filtering 10Hz-1KHz 20Hz-15KHz
ADC accuracy 14 16
Input / output port 1TTL I / O
3 analog output
4TTL I / O
4 analog outputs







product type:
Ø 3 channels: output via analog signal
Ø 4 channels: full configuration channel, integrated biosensor, reconfiguration through signal conversion of preamplifier
The 4-channel system has all the features of a 3-channel system. In addition, a channel is added to configure a flexible synchronous biosensor.


Optional accessories:
Ø Biosensor
Ø Integrated video synchronous recording equipment


Brain chemical real-time monitoring module:

环伏安 法(FSCV)电化学检测系统,用于儿茶酚胺(ie, dopamine, norepinephrine, and serotonin)的测量。 Fast scanning cyclic voltammetry (FSCV) electrochemical detection system for the measurement of catecholamines (ie, dopamine, norepinephrine, and serotonin). Can be used to measure animals in free awake state. The telemetry system is suitable for mice.


    FAST SCAN CYCLIC VOLTAMMETRY SYSTEM

    Pinnacle's FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is specifically designed to simplify the measurement of catecholamines (ie, dopamine, norepinephrine, and serotonin). Pinnacle offers turn-key systems for both mice and rats. Both the wireless rat system and the tethered mouse system use carbon fiber sensors to enable short-term measurement in the brains of freely moving animals .




      HOW IT WORKS 

    Biogenic amine levels are detected by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resulting current. Our systems can measure spontaneous sub-second neurotransmitter release events while conducting detailed behavioral studies. Both the wireless and tethered systems sweep from 250 to 400 V / s in a user-selectable range spanning -1.1 to +1.3 V. All systems have built-in support for controlling an external stimulus.

    l Voltage Span: -1.1 V to +1.3 V

    l Range: 250 – 400 V / s

    l Sweeps / second: 5-10

    l Points / sweep: 1000



    Analysis

    Dopamine (blue), serotonin (green), and norepinephrine (pink) have specific voltammogram profiles when detected by FSCV.


    The TETHERED FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM allows researchers to harness the powerful genetics of the mouse model to address underlying mechanisms of biogenic neurotransmitter release and function. A headmounted FSCV board sends signals through a low-torque commutator to an interface box that streams data to the host PC. The system comes with Pinnacle's FREE 8500 software.



    1. The FSCV interface box provides access to stimulus lines and transmit data to PAL-8500 software.

    2. A low-torque commutator, which is mounted above the cage, allows for unencumbered freedom of movement.

    3. Signals are amplified and filtered at the head of the animal using our headstage, which ensures the delivery of clean, artifact-free data.

    4. Stereotaxically placed guide cannulas allow easy insertion of carbon fiber sensors. Headmounts are affixed to the skull with dental acrylic and act as a connection port for the headstage.




    CARBON FIBER


    Pinnacle's FSCV system uses CARBON FIBER SENSORS (CFSs) to measure the presence of dopamine and other catecholamines in the brains of freely moving animals. Our sensor is a 34 µm diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica. All Pinnacle CFSs require an Ag / AgCl reference electrode.


    Our sensor is a 34 um diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica.

     




    CARBON FIBER SENSORS

    Carbon fiber sensors are ordered by cannula type and whether the researcher needs to remove them from the cannula for post-calibration. CFS-F sensors are fixed in the cannula and cannot be removed for post-calibration.



    FSCV PAL-8500 SOFTWARE PACKAGE

    The FSCV system includes Pinnacle's FREE PAL-8500 software, which supports traditional, short recording paradigms (recordings of two minutes or less) as well as longer-term recordings that use an extended continuous mode. Furthermore, the suite supports integrated, synchronized video recording , which allows monitoring of animal behavior simultaneously with biogenic amine release.


    Additional features of this software include:
    • Background Subtraction
    • 3D Visualization
    • User-Selectable Filters
    • Heat Maps
    • Animated Voltammograms
    • Export to Third-Party Packages




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