⎝⎛七乐彩⎞⎠

Shanghai Yuyan Scientific Instrument Co., Ltd.

Phone: 021-35183767
021-54377179

FAX: 021-35183767-8008

QQ: 3007536621

Address: XingMei Rd 485, Shanghai, China, No.485 Xingmei Road, Minhang District, Shanghai

Location: ⎝⎛七乐彩⎞⎠ > Cell Culture and Analysis Equipment> Microfluidic Cell Workstation
Cell culture and analysis equipment

Microfluidic cell workstation

来源:本站 作者:玉研仪器 Time: 2016-12-16 Source: Site Author: Jade research instrument

Detailed introduction

TM 微流体 细胞 工作站 目前非常受客户欢迎的一套系统,提供在活细胞水平上微流体活性筛选的完整解决方案。 The VenaFlux TM Microfluidic Cell Workstation is currently a very popular system for customers, providing a complete solution for screening microfluidic activity at the living cell level. Simple operation and high analysis efficiency can greatly improve the efficiency of drug development and screening.


The VenaFlux system is the first semi-automated microfluidic analysis system capable of continuous fluid cell analysis. The Cellix biochip and cell analysis software can be used to detect cells attached to the microcapillary. The microcapillary is covered with antibodies or endothelial cells are cultured to simulate a physiological flow to generate a certain shear force. The VenaFlux system is simple to operate, which can reduce time-consuming and expensive animal model experiments, and obtain results with strong specificity, high accuracy and good repeatability.

 

Product Features of VenaFlux Microfluidic Cell Analyzer


广泛使用于各种细胞悬浮液 Ø Widely used in various cell suspensions ;

生物芯片可选择用光学显微镜做细节研究 Ø Biochip can choose to use optical microscope for detailed research ;

微流体泵可方便的控制流体剪切力在0.05-200 Dyne/cm2范围内 Ø The micro fluid pump can conveniently control the fluid shear force in the range of 0.05-200 Dyne / cm2 ;

在分析中可调整剪切力 Ø Shear force can be adjusted during analysis ;

流体速度可控制在pL/min到mL/min Ø Fluid speed can be controlled from pL / min to mL / min ;

可做nL到mL 的样品分析 Ø Can analyze samples from nL to mL ;

使用DucoCell 分析软件(DucoCell Analysis Software),可简单快速的调节好显微镜的各项参数 ØUsing DucoCell Analysis Software, you can quickly and easily adjust microscope parameters

统计分析生成的图表(如:细胞粘附性VS.剪切力)可方便的导入Excel文档 Ø Charts generated by statistical analysis (such as cell adhesion vs. shear force) can be easily imported into Excel files ;

电子机械臂移动生物芯片,清洗细微组件和稀释细胞样品 Ø The electronic mechanical arm moves the biochip, cleaning the micro components and diluting the cell sample ;

VenaFluxTM 系统的软件同步调节泵,翻译阶段筛选,图像采集,样品移动和流体控制,各项操作界面均是用户友好型,系统先进又易于操作 Ø The software of the VenaFluxTM system adjusts the pump synchronously, screens translation stages, image acquisition, sample movement and fluid control. All operation interfaces are user-friendly, the system is advanced and easy to operate ;


配置方案: Main configuration scheme:

Partial Platform -STARTER-1.0: I. Partial Platform -STARTER-1.0:

  1.Mirus Nanopump 1.0
2.1 pack VenaEC biochips
3.1 pack Vena8 biochips
4.Frame for VenaEC biochips
5.DucoCell Software ver 1.1


Microfluidic SP1.0 (Basic full Package) -MICROSP-1.0:

1.arl Zeiss Axiovert 40 microscope, CCD Deltapix DP200 camera, Dell PC, Microscope cage incubator

2.Mirus Nanopump 1.0 inc. VenaFluxAssay software
3.1 pack Vena8 biochips
4, 1 pack VenaEC biochips
5.DucoCell Software ver 1.1


CMicrofluidic SP1.1-MICROSP-1.1:

1. Carl Zeiss Axiovert 40 CFL microscope, CCD Deltapix DP450 camera, Dell PC, Microscope cage incubator, Fluorescent and brightfield shutters,
2.Mirus Nanopump 1.0 inc. VenaFluxAssay software
3.1 pack Vena8 biochips
4.1 pack VenaEC biochips
5.DucoCell Software ver 1.1


Microfluidic SP1.2-MICROSP-1.2: Fourth, Microfluidic SP1.2-MICROSP-1.2:

1. Carl Zeiss Axiovert 40 CFL microscope, CCD Deltapix DP450 camera, Dell PC, Microscope cage incubator, Fluorescent and brightfield shutters, motorised

stage
2.Mirus Nanopump 1.0 inc. VenaFluxAssay software
3.1 pack VenaEC biochips
4.1 pack Vena8 biochips
5.DucoCell Software ver 1.1


V. VenaFlux-VENAFLUX-1.0:

Carl Zeiss Axiovert 40 CFL microscope, CCD Deltapix DP450 camera, Dell PC, Microscope cage incubator, Fluorescent and brightfield shutters 1. Carl Zeiss Axiovert 40 CFL microscope, CCD Deltapix DP450 camera, Dell PC, Microscope cage incubator, Fluorescent and brightfield shutters
2.VenaFluxAssay software plus automatic image capture
3.Robotic sample handler
4.Mirus Nanopump 1.1 inc. 8-way Manifold
5.1 pack VenaEC biochips
6.1 pack Vena8 biochips
7.Frame for VenaEC biochips
8.DucoCell Software ver 1.1



The main technical parameters:

Microscope Axiovert 40:
Contrast option: Brightfield, Phase, Varel.
Objectives: 10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat, 40x LD A-Plan
Eyepieces: E-PL 10x / 20 Br., 10x / 20 Br. Foc.
Stage: Vena8 biochip compatible stage holder, Eppendorf tubes holder.


Mirus 1.0 Nanopump
Specifications:   Shear Stress Range of 0.05–20 dyne / cm2,

Volumetric Flow Rates 100nL--20μL / minute (100μL syringe),

Sample Volume Increments Freely adjustable   Linear Velocity Range of 10 μm / s to 10 cm / s;

Flow Direction Reversible;

Sample Volume Aspiration Accuracy ± 1%;

Shear Stress Accuracy ± 0.5%;

Sample Volume Aspiration Precision <1%.
Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.


Microscope Cage incubator:
Capabilities: Temperature control ± 0.10C, CO2 and humidity module.
Options: Temperature monitoring software module, black panels for use in fluorescent microscopy.


Deltapix DP 200 USB 2.0 camera
Specifications: 1.3 Mega Pixels (1,280 x 1,024), Color CDD, 1/2 "format, Pixel 5.2 μm x 5.2 μm,

Resolution: 15 FPS @ 1,280 x 1,024 pixels; 24 FPS @ 1,204 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range> 60 dB.


Dell Optiplex 745 PC computer
Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO;

Objective Imaging stage control hardware; 19 ”LCD monitor.

Windows XP Professional SP2;

DeltaPix Viewer Software;

FlowAssay and DucoCell software preinstalled.


FlowAssay Software.
Capabilities: Execution of preset protocols.

Intuitive stepwise protocol interface with steps hierarchy and status control.

 

DucoCell software
Specifications: Automatic Counting and Detection;

Morphological parameters analyzed automatically (Area, diameter, form-factor, ellipticity, eccentricity, orientation, perimeter, asymmetry, cell centroid coordinates, elliptical axis);

Cell sorting and filtering;

Ability to analyze sub-populations based on inclusion / exclusion of morphological parameters.

Data may be exported to Excel spreadsheet incorporated automatic graph generation (eg% cell adhesion vs. shear stress).

File Formats analyzed * .jpg, * .jpeg, * .tif, * .tiff, * .bmp, * .gif, * .png.


Vena8 biochips pack of 10

Specifications: Minimum Sample Volume 3 μL;

Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne / cm2, steps of 0.05dyne / cm2.

Number of channels per biochip is 8;

Volume of each channel is 0.8 μL.

Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm (L).


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