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In vitro tissue experiment

Auraora Animal Muscle Test System

来源:本站 作者:玉研仪器 Time: 2018-07-12 Source: Site Author: Jade research instrument

Detailed introduction

The Auraora animal muscle test system can test the strength of an animal's peeling muscle cells, a muscle fiber, a muscle, or even a whole group of muscles. It measures the mechanical characteristics of a single intact muscle, allowing experimenters to change Interpret the mechanical force index of the muscle from an angle. It is specially designed for a variety of animals such as mice, rats, rabbits, etc. It has a variety of models for in vitro muscle test and in vivo test, and has accumulated a large number of references in related fields. It is the gold standard for muscle test research.

 
The isolated muscle test system is specifically designed for mice, which can test the contractile strength and different mechanical characteristics of animal (rat and mouse) muscles. From simple isometric twitch schemes to more complex force-contraction rate schemes, various mechanical parameters of the muscles (contraction strength, length change, contraction rate, etc.) are accurately tested.
 
小肌肉(如FDB和蚓状肌),甚至是一些人造肌肉,聚合物或者是薄膜。 In-vivo muscle testing can measure the mechanical properties of a certain muscle in an animal. It can be applied to the muscles of animal limbs, such as long extensor toes, tibialis anterior muscle, soleus muscle, gastrocnemius diaphragm muscle, small muscles (such as FDB And vermiform muscles), or even some artificial muscles, polymers, or membranes.


The muscle test system also has a test system specifically for single myofibrils, muscle cells, muscle bundles, single muscles or small muscles such as trabeculae. Using the two methods of atomic force microscope and beam deflection, the kTR, force-calcium ion concentration of muscle fibers , Mechanical properties such as length-tension, force-shrinkage rate and hardness.

During the test, the device probe is connected to the tendon at one end of the muscle to test and control its strength and length. During the experiment, a single muscle will be fixed in an environment that supplies oxygen and nutrient solution, and two electrodes are placed on both sides of the muscle to provide stimulation. The position adjuster can put each component in a better position, and record and analyze all data through dynamic muscle control and analysis software (DMC / DMA). Various parameters such as quietness, resting length, stimulation frequency and actual test schemes can be set using software, and the system can also provide some standard experimental schemes for reference.


主要特点: Main features of animal muscle test system :


The muscle test system can measure the mechanical characteristics of a single intact muscle, with a peak intensity of 10N / 1000grams; a resolution of 1nm;
Have different test plans: such as isometric twitch, tonic contraction, fatigue, injury, length-tension, contraction rate, stimulation frequency, hardness, work cycle, etc .;
It can be applied to the muscles of animal limbs, such as long toe extensor, tibialis anterior muscle, soleus muscle, gastrocnemius diaphragm muscle, small muscles (such as FDB and vermiform muscle), and even some artificial muscles, polymers or membranes. .


离体无损肌肉测试系统 1200A Isolated Non-Destructive Muscle Test System

You can test muscle contraction and different mechanical properties. The test process only needs to connect the tendon at one end of the muscle to test and control its strength and length, which greatly reduces the operation time, and also improves the efficiency and output of the experiment.


如趾长伸肌﹑ 胫骨前肌﹑ 比目鱼肌﹑ 腓肠肌﹑ 隔膜肌﹑細小肌肉 ( FDB 和蚓状肌 ) , 甚至是一些人造肌肉, 聚合物或者是薄膜。 This system can be applied to the muscles of animal limbs, such as long toe extensor, tibialis anterior muscle, soleus muscle, gastrocnemius muscle, diaphragm muscle, small muscles ( such as FDB and vermiform muscle ) , and even some artificial muscles, polymers or film.

单条肌肉会直立地固定于一个供应氧气和营养液的容器之中, 在肌肉两侧会有两条电极提供刺激。 During the preparation process, a single muscle will be fixed upright in a container supplying oxygen and nutrient solution, and there will be two electrodes on both sides of the muscle to provide stimulation. The position adjuster can make each part in a good position, from placing muscles to requesting experimental data, so that the entire experimental pipeline is completed.


(DMC/DMA) , 能 实验方案设定去到数据收集和分析,大大缩短整个实验过程的需时, 甚至于数分钟内完成。 Through dynamic muscle control and analysis software (DMC / DMA) , it is possible to move from experimental protocol setting to data collection and analysis, greatly reducing the time required for the entire experimental process, even within minutes. 静息长度, 刺激频率和实际测试的方案都可以使用软件去进行设定, Various parameters such as quietness, resting length, stimulation frequency and actual test scheme can be set using software.  
Samples of some standard protocols will be provided in the system.


原位 / 体内 / 离体大小鼠肌肉测试系统 1300A in situ / in vivo / ex vivo muscle test system

特点 Main features :
高效的三合一系统设计 : Efficient three-in-one system design :
原位实验 2) 活体动物体内实验 ( 踏板设计 ) 3) 离体实验 ( 浴盆 ) 1) In situ experiments 2) In vivo experiments in living animals ( pedal design ) 3) In vitro experiments ( tub )
适用于损伤和纵长等课题的活体动物实验 Live animal experiments suitable for subjects such as injury and length
可选择 25ml 浴盘应用于离体肌肉实验 25ml bath tray can be selected for isolated muscle experiments
操作简单的高产量测试系统, 适用于任何经验水平的研究者 Simple operation and high-yield test system, suitable for researchers of any experience level


三合一测试系统的设计主要是让肌肉生理学家能够通过简易的操作去测试出大鼠或小鼠等肌肉组织的机械特性和功能参数。 The design of the 1300A three-in-one test system is mainly to allow muscle physiologists to test the mechanical characteristics and functional parameters of muscle tissues such as rats or mice through simple operations.
拥有一个独特的双模肌肉杠杆系统 , 通过连接着肌肉其中一端肌腱的基础上, 同时进行肌肉在收缩力度和长度变化两方面的测量和控制,这大大地节省了实验需时和提高了实验的效能。 1300A has a unique dual-mode muscle lever system . By connecting the tendon at one end of the muscle, it can simultaneously measure and control the muscle in terms of contraction strength and length changes, which greatly saves time and improves the experiment. Performance.
疲劳, 硬度, 力度 - 收缩速率和功循环等实验方案都可以直接应用于 1300A 测试系统, 同时具有高弹性尺度, 可以应用於等距测试方案, 例如抽搐, 强直收缩和力量 - 刺激频率等实验方案。 Elongational contraction, fatigue, hardness, force - contraction rate, and work cycle can be directly applied to the 1300A test system. At the same time, it has a high elastic scale and can be applied to isometric test programs, such as convulsions, tonic contraction, and force - stimulation. Frequency and other experimental protocols.


透性肌纤维测试系统 1400A Permeable Muscle Fiber Test System

特点 Main features :
力度峰值达至 100mN Strength peak reaches 100mN
kTR , 力 -pCa , 长度 - 张力, 力 - 收缩速率,和硬度等实验方案都可以应用于该系统 KTR , force- pCa , length - tension, force - shrink rate, and hardness can be applied to the system
可控温浴盘具有八个溶液小槽以盛载不同浓度的钙离子溶液, 并且能夠自动化地进行溶液小槽间的转换 ● The temperature-controlling bath plate has eight small solution tanks to hold different concentrations of calcium ion solutions, and can automatically switch between solution small tanks
温度控制范围 : 0-40 °C Temperature control range : 0-40 ° C
可以配合标准倒置显微镜使用 Can be used with standard inverted microscope


测试系统设计理念始于简化去膜纤维实验的操作,同时提高实验数据的输出。 The design concept of the 1400A test system begins with simplifying the operation of the membrane removal fiber experiment and improving the output of experimental data. 该系统的应用将去膜纤维实验实现化。 This system has the characteristics of one-stop experimental operation. Whether it is a leading expert or a backward researcher, the application of the system can realize the film removal fiber experiment. 以進行肌小節間隔測量和控制。 The experimental device of this system is used with an inverted microscope to measure and control the sarcomere interval. 8 個溶液小槽的移動方位和次序。 The revolutionary experimental device is accompanied by a software application. Through a unique program , the movement position and sequence of the eight solution tanks can be controlled . This sequencer allows researchers to automate the positioning of the bathtub under the prior instructions, and has the space to synchronize with other key steps.
测试系统可以控制和测试力度,长度和肌小节的间隔长度(当使用可附加的高速视频肌节长度 1400A test system can control and test the strength, length and sarcomere interval length (when using attachable high-speed video sarcomere length
使其应用范围更广泛,从简单的力 - 钙离子浓度曲线去到完整的组织机械特性(例如 System) to make it more versatile, from simple force - calcium ion concentration curves to complete tissue mechanical properties (such as
, 長度 - 張力,力 - 收縮速率,和硬度等 ) 都能够進行测试。 KTR , length - tension, force - shrinkage rate, and hardness, etc. can be tested. (600A) 备有各种标 Additional software program (600A) with various standards
Standard experimental scheme to meet different experimental needs.


细小组织测试系统 1500A fine tissue test system

main feature
设计应用于肌纤维, 肌肉束,单条肌肉或细小的肌肉如 trabeculae Designed to be applied to muscle fibers, muscle bundles, single muscles or small muscles such as trabeculae
适用于长度 - 张力, 力 - 收缩速率和硬度等的测量 Suitable for measuring length - tension, force - shrinkage rate and hardness
力度峰值达至 100mN Strength peak reaches 100mN
可控温度范围 : 0-40°C Controllable temperature range : 0-40 ° C
400μL 1900μL 循环灌流浴, 包括两条铂电极用以提供刺激 400μL or 1900μL circulating perfusion bath, including two platinum electrodes to provide stimulation
可以配合标准显微镜或者倒置显微镜 Can be used with standard microscope or inverted microscope
可选择单一等距实验模式 Select single isometric experiment mode


It can make it easier and faster for scientists engaged in physiological research to test the mechanical properties of small tissues, such as universal force parameters. 应用于成像观察和萤光测量。 The experimental device of this system is matched with most of the inverted microscopes, which are used for imaging observation and fluorescence measurement. The experimental device has two different size bath trays to choose from for small or medium size samples. The temperature-controllable bathtub is equipped with a solution circulation perfusion device and two built-in platinum electrodes to provide different types of electrical stimulation. 1L 水库为浴盆供应含氧媒体。 The system is also equipped with a 1L reservoir to supply oxygen-containing media to the bathtub.
该设计令浴盆的底部可以被有机玻璃密封, 从而非常适用于进行人工心肌组织或一些高氧耗量的研究。 The force sensor is connected to the sample tissue through a small groove at one end of the bathtub. This design allows the bottom of the bathtub to be sealed by plexiglass, which is very suitable for artificial myocardial tissue or some high oxygen consumption research. 1500A 是一个非常理想的测试系统去应用于细小肌肉组织方面的实验。 Because the configuration is very limited, the 1500A is an ideal test system for experiments on fine muscle tissue.


透性肌肉细胞测试系统 1600A Permeable Muscle Cell Test System

main feature
应用于透性 ( 去膜 ) 心肌细胞的研究 Research on the application of permeable ( stripping ) cardiomyocytes
测试 kTR, - 钙离子浓度 , 长度 - 张力 , - 收缩速率和硬度等机械特性 Test mechanical properties such as kTR, force - calcium ion concentration , length - tension , force - shrinkage rate and hardness
备有 8 个溶液小槽的寛阔平台以盛载不同浓度的钙离子溶液 A wide platform with 8 small solution tanks to hold different concentrations of calcium ion solutions
电动化三维微定位装置以准确和快速地将样本定位 Motorized three-dimensional micro-positioning device to accurately and quickly locate samples
可以配合标准显微镜或者倒置显微镜 Can be used with standard microscope or inverted microscope
可控温度范围 : 0-40°C Controllable temperature range : 0-40 ° C


是一个可靠和可重复性的实验测试系统,应用于测试去膜心肌细胞的主动和被动性质。 1600A is a reliable and reproducible experimental test system for testing the active and passive nature of membrane-deprived cardiomyocytes. By using its joystick to control the motorized positioning device, the two ends of the cell can be easily connected to a force sensor and a high-speed servo motor, which greatly improves the experimental performance and simplifies the original fine sample connection procedure.
8 个溶液小槽令细胞可以轻易地在不同浓度的钙离子溶液之间进行转换,该装置会直接放置在倒置显微镜的置物平台上进行高清晰度的观察和肌节间距的检测。 The temperature-controllable bathtub has 8 small solution tanks to allow cells to easily switch between different concentrations of calcium ion solution. The device will be placed directly on the platform of an inverted microscope for high-definition observation and sarcomere pitch Detection. You can choose to attach a prism to accurately detect the depth of cells in the solution tank.
测试系统可以控制和测试力度, 长度和肌小节的间隔长度 ( 当使用可附加的高速视频肌节长度系统 ) ,使其应用范围更广泛, 从简单的力 - 钙离子浓度曲线去到样本完整的机械特性 ( 例如 kTR ,长度 - 张力, 力 - 收缩速率和硬度 ) 都能够被测试。 The 1600A test system can control and test the strength, length and interval length of the sarcomere ( when using the attachable high-speed video sarcomere length system ) , making it more widely used, from simple force - calcium ion concentration curve to complete sample Mechanical properties ( such as kTR , length - tension, force - shrinkage rate, and hardness ) can be tested. (600A) 备有各种标准的实验方案去配合不同的实验需求。 The additional software program (600A) is equipped with a variety of standard experimental protocols to meet different experimental needs.


( 企鹅系统 ) Myofibrillar Test System ( Penguin System )

main feature
利用不同的实验方案去测量肌原纤维的各种机械特性和功能表现 Using different experimental schemes to measure various mechanical characteristics and functional performance of myofibrils
具可控温的组织浴盆 Tissue tub with temperature control
可配合倒置显微镜同步使用 Can be used simultaneously with an inverted microscope
具缓冲液置换系统,用以设定肌小节的长度和肌原纤维的快速兴奋作用 With a buffer replacement system to set the length of the sarcomere and the rapid excitatory effect of myofibrils
分辨率 : 1 nN ( 納米 ) Resolution : 1 nN ( nm )
力度的测量范围 : 1-10 μN ( 取决於使用的悬臂 ) Measuring range of force : 1-10 μN ( depending on the cantilever used )


( 纳米范围 ) ,亦是市场上第一套针对测量肌原纤维的商业系统。 The appearance is similar to penguin's myofibrillar test system, which is specially designed to measure the tiny force ( nano range ) generated during the activation of myofibrillar . It is also the first commercial system for measuring myofibrillar on the market. : 一个力传感器 ( 可以固定於倒置显微镜的後悬臂 ) ,一个具有可控温浴盆的平台,原子力显微镜悬臂和一枝可调位的玻璃针。 The main components of this test system are : a force sensor ( can be fixed to the rear cantilever of an inverted microscope ) , a platform with a temperature-controllable bathtub, an atomic force microscope cantilever, and an adjustable glass needle. The configuration of the system makes it easy to carry out different experimental schemes, such as the relationship between detection force and calcium ion concentration, relaxation test, relationship between detection hardness and detection force and shrinkage rate, and so on. At the same time, the two functions of sarcomere length measurement and myofibrillar imaging can be integrated into this system.


(AFM) 和光束偏转 (OBD) 两大方法。 The operation of the penguin system is based on the application of atomic force microscope (AFM) and beam deflection (OBD) . When the myofibrils produce contractile force, the cantilever has a slight deflection, and then the laser is used to measure its deflection to calculate the strength. AFM 悬臂和玻璃针之间。 During the experiment, myofibrils will be connected between the AFM cantilever and the glass needle. 悬臂连接着力传感器去测量肌原纤维的力度变化,而玻璃针连接着长度操控仪去控制和改变肌原纤维的长度,从而能够完整地描述肌原纤维的功能表现和机械特性。 The AFM cantilever is connected to a force sensor to measure the strength change of myofibrillar fibers, and the glass needle is connected to a length manipulator to control and change the length of myofibrillar fibers, so that the functional performance and mechanical characteristics of myofibrillar fibers can be completely described.




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