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How to Select Hypoxic Experiment Models Correctly

来源:本站 作者:玉研仪器 Time: 2019-11-11 Source: Site Author: Jade research instrument

In laboratory research, when it comes to hypoxia studies such as myocardial ischemia, reperfusion, and apnea syndrome, it is often necessary to perform hypoxia modeling on experimental animals. What kind of hypoxia model is created at this time and how to create it are particularly important.

The hypoxia model is mainly divided into hypoxia modeling under normal pressure environment and hypoxia modeling under low pressure environment. Some teachers may be confused at this time. What kind of environment should I choose for modeling? What kind of model does it correspond to?

常压环境低氧,体积分数改变,低压环境低氧,质量分数改变, 那么就能够灵活的判断自己所需的实验类型了。 In fact, we only need to remember one principle of concentration change: low oxygen in normal pressure environment, change in volume fraction, low oxygen in low pressure environment, change in mass fraction, then we can flexibly judge the type of experiment we need.

 

Hypoxia in the atmospheric environment, referred to as atmospheric oxygen for short, is due to the lack of oxygen caused by different volume fractions to model experimental animals.

Hypoxia in a low-pressure environment, referred to as low-pressure oxygen, is caused by the lack of oxygen caused by different mass fractions to model experimental animals.

 

N 2 :O 2 =4:1 The ratio of normal air under normal pressure environment is roughly divided into the volume ratio N 2 : O 2 = 4: 1

For low oxygen in low pressure environment, the air ratio is still roughly the volume ratio within a sub-volume; N2: O2 = 4: 1, but the mass is reduced, that is, the mass of the gas in a sub-volume is reduced, and the actual volume ratio No change


Hypoxia under atmospheric pressure is actually a change in the volume ratio of N2 and O2 in a subvolume, resulting in a change in the concentration in the volume fraction.

Hypoxic mode, the volume ratio of the two in a subvolume changes

 

Nitrox mode, the volume ratio of the two in a subvolume changes

 

Therefore, as long as the teachers know whether the concentration change that requires a change in volume ratio or the concentration change that requires a change in volume ratio, they can better judge the type of experiment required.

 

And Yuyan's hypobaric oxygen system uses the principle of simulating the altitude of the plateau to achieve research needs in different directions. Functional and structural effects) and other experimental studies. The instrument uses a vacuum pump to simulate low pressure differences caused by different heights for low pressure experiments at different height environments. The higher the altitude, the lower the air pressure, so when simulating different altitudes, you only need to make the pressure of the instrument correspond. The difference in altitude causes a decrease in the mass fraction of oxygen in the air, not a decrease in the volume fraction. Therefore, the volume fraction is still 21% at different altitudes, but the hypoxia caused by the change in mass fraction.


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