Address: XingMei Rd 485, Shanghai, China, No.485 Xingmei Road, Minhang District, Shanghai
50 mice, 1% sodium pentobarbital solution, mouse cage, balance, 0.5ml or 0.25ml syringe.
Third, the experimental steps
1. Determine the dosing dose: pre-experiment with a small number of animals to obtain the minimum dose of 100% hypnotic response to sodium pentobarbital (ED100)
And the response rate was 0% of the maximum dose (ED0). Then within this dose range, it is divided into several dose groups (usually 4 to 8 groups) according to the proportional series.
The common ratio (r) of the dose in each group is r = n-1√ ED100 / ED0
After finding r, multiply r by starting from the first dose group (ED0) to get the dose of the next next group. If divided into 5 groups,
Do not specify ED0, r · ED0, r2 · ED0, r3 · ED0, r4 · ED0, r5 · ED0.
2. Dosing: Take 50 healthy mice weighing about 25g, check the random number table, and randomly divide them into 5 groups, 8 in each group (except for extreme small weight)
2 rats). 0.1ml / 10g was injected intraperitoneally according to the concentration of each group listed in Table 1.
3. Recording results: Take the disappearance of righting reflex as the index of falling asleep, observe the hypnotic effect of the drug, and record the number of sleeping rats within 15 minutes after intraperitoneal injection.
Fill in Table 1.
4. Experimental results
Table 1 Calculation table for pentobarbital sodium ED50
According to the data in Table 1 and the above formula, ED50 = 31.90
1. To reduce errors, dispense medicine by 1 person
2. Keep the environment quiet
3. If positive animals appear in the first group, increase the low-dose group by the common ratio (d0 = d1 / r)
4. The animals in the last group are not all positive, increase one dose upward (dk + 1 = dkr)
Anesthesia methods for experimental animals, in addition to using sodium pentobarbital for injection anesthesia, can also use isoflurane gas anesthesia:
Small animal gas anesthesia machine
Animal insulation system
对手术中的动物和术后恢复的动物进行保温，并可进行动物体温测量。 The animal body temperature maintaining instrument (electric heating type) keeps warming the animals during and after the operation, and can also measure the body temperature of the animals.
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