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(1) Tail tip blood collection: This method is used when the required blood volume is small. Fix the animal and trace the mouse tail, soak the mouse tail in warm water at 45 ° C for several minutes, or rub it with chemical drugs such as xylene to expand the local new blood vessels. Dry the mouse tail, cut off the tail tip, and the blood flows out of the tail tip, let the blood drip into the receptacle or directly suck with a pipette. If you need to take a certain period of time, take the blood from the tip of the rat's tail several times. When you take blood, cut off a small section of the tail. After taking the blood, first use a cotton ball to stop the bleeding and immediately apply 6% liquid collodion to At the wound of the tail, a layer of collodion film is formed outside the wound to protect the wound. Blood can also be cut by cutting the tail vein. Three tail potential veins can be cut alternately and cut from the tail tip to the tail root. 0.2 ~ 0.3ml blood can be taken each time. After cutting, the cotton is used to stop bleeding. This method of blood collection is better in rats, and continuous blood can be taken at longer intervals for routine blood tests.
(5) Blood extraction from the heart: Animals are supine and fixed on a fixed plate, the coat on the anterior heart area is cut off, and the skin is disinfected with iodine alcohol. In the 3rd to 4th intercostal space on the left, use your left index finger to touch the heart beat, and take a syringe with a 4 to 5 gauge needle in your right hand, and choose the puncture where the heart beats the most. When the needle penetrates the heart, the blood will beat because of the heart Power into the syringe automatically. This method requires the experimenter to grasp the following points: insert the heart quickly and directly, otherwise, the heart will slip off from the needle tip; if the needle is not punctured for the first time, remove the needle from the spine and avoid probing around the heart, so as not to damage the heart, Lungs; suck slowly and steadily, otherwise, too much vacuum will collapse the heart. If you don't need to save the life of the animal, you can also numb the animal's chest after intoxication, and pierce the syringe directly into the human heart to suck blood.
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